Yogi Vemana University M.Sc Degree Zoology Third Semester , November 2016

M.Sc. DEGREE EXAMINATION, NOVEMBER 2016.

THIRD SEMESTER

Zoology

Paper II -- MOLECULAR BIOLOGY

Time: Three hours                                                                                    Maximum 75 marks

(No additional sheet will be supplied)

PART A -(5 x 3 = 15 marks)

Answer any FIVE questions.

Each question carries 3 marks.

Each answer should not exceed 1 page.

1. Central dogma of Molecular Biology

2. One gene one enzyme hypothesis.

3. Reverse Transcriptase

4. DNA Polymerase I

5. Ribozymes

6. Inhibitors of translation

7. Tryptophan operon

8. Sanger dideoxy method of sequencing.

PART B -(4 x 5 = 60 marks)

Answer ALL questions.

Each question carries 15 marks

Each answer should not exceed 6 pages.

9. Give a detailed account of Watson and Crick model of DNA and properties of DNA.

10. Write a note on :
(a) Nuclear organisation and
(b) Mitochondrial Genome

11. Write in detail about different DNA repair mechanisms and add a note on their significance in maintaining DNA integrity.

Or

12. Write a detail note on various enzymes involved in DNA replication with special emphasis on their function.

13. Write a note on different types of RNA and their functions

Or

14. write a detailed note on
(a) Transcription and
(b) Post transcriptional modifications.

15. Write detail note on gene regulation with special reference to Lac operon.

Or

16. Write a detailed note on construction of cDNA libraries.