Yogi Vemana University III rd B.Sc Biotechnology (Molecular Biology, Genetic Engineering & Immunology) 2012 Question Paper

Govt. Degree College, Kodur(RS), Kadapa (Dist)

Affiliated to SV University, Tirupati.

THREE YEAR B.Sc. DEGREE EXAMINATIONS Pre Final 2012

Third Year Examination

Part ll - Biotechnology

Paper III-Molecular Biology, Genetic Engineering & Immunology

Time: 3 hours                                                                                                    Max. Marks: 100

SECTION - I

Answer all questions. Each question carries 15 marks.                                     (Marks: 4 x 15= 60)

1. (a) Explain the mode of DNA replication with the help of Messelson & Stahl experiment.

(or)

(b) Explain the formation of TT dimmers and different types of repair mechanisms involved

2. (a) Describe the mechanism of initiation and elongation of transcription.

(or)

(b) Explain Gene regulation with the help of trp operon.

3. (a) Explain the different type of vectors used in r DNA technology.

(or)

(b) Explain the different methods of introduction of r-DNA into host cells.

4. (a) Write an account on cells of the immune system

(or)

(b) What is Major histocompatibility complex. Explain its role in organ transplantation.

SECTION - Il

Answer any EIGHT questions. Each question carries 5 marks.                          (Marks: 8 x 5 =40)

5. Klenow fragment.

6. Origin of replication.

7. Telomeric DNA.

8. RNA Polymerase.

9. Termination of transcription.

10. Post transcriptional modifications

11. PCR.

12. Restriction endonucleases.

13. Southern blotting

14. Hapten.

15. Antigen Antibody reactions.

16. Hyper sensitivity